Fibrillar Monolith FAQs
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What is fibrillar polymeric monolith technology and how does it differ from traditional polymeric monolithic technology?
What mixtures can be separated with these columns?Because of the large pore size and reduced surface area, the FM-PhEs columns are not recommended for small molecule analysis. However, this structure is well suited for analysis and purification of proteins and other large molecules. Before using the columns, what pre-conditioning is suggested?Invenca HPLC columns are shipped in 30% methanol. Equilbration with your mobile phase is recommended until a stable baseline is observed. Is there a flow direction?The flow direction is indicated by an arrow on the column label. Will pressure or impact shock damage or impair the column?General lab mishaps, like dropping the column on the floor, will not damage the column packing. In use, dramatic pressure jumps should be avoided on general principle. Can the columns be autoclave sterilized? How about chemical sterilization?Autoclave sterilization is not recommended. Chemical sterilization with dilute sodium hypochlorite or sodium hydroxide is acceptable. What pressure limits should be avoided or have been tested?The FM-PhEs HPLC columns are recommended for use below 5000psi. How should I store and handle the columns – are there any unusual precautions?FM-PhEs HPLC columns may be stored in your mobile phase while in use, although we recommend at least 15% organic content to prevent biofilm formation. For extended storage periods, storage in 30% methanol or isopropanol is recommended. What solvents should be avoided?Halogenated solvents can cause the column packing to swell and should be avoided. Primary amines will degrade the column packing at temperatures above 40°C. Strong oxidizing agents,such as concentrated sulfuric acid, will cause irreversible damage. What is the operating temperature range?FM-PhEs columns can be used up to 80°C. At low temperatures, it is important to avoid conditions that might cause the mobile phase to freeze in the column. Care should be taken to allow the columns to fully equilibrate, especially at elevated temperatures. How should they be cleaned or rejuvenated?Invenca FM-PhEs HPLC columns may be cleaned with:
What range of sample loadings can be accommodated?Because of the large pore sizes, FM-PhEs media have lower surface area than traditional porous particle-based media, so you may experience reduced loading capacities. Will 100 % aqueous mobile phase harm the column?No. The FM-PhEs surface is not derivatized and will not suffer from phase collapse. If salts have precipitated in the column, how should they be removed?Back-flushing with warm water or other suitable (and column compatible) solvent is suggested. |
Polymeric fibrillar monolith technology creates controlled pore shape and orientation for chromatography, filtration and other extractive media. Fibrillar monoliths are composed of thousands of oriented polymeric microfibrils that are partially fused together. This enables fewer blind and eddy spots than granular, nodular and spherical based packings. Clogging, blinding, shock damage and sample carry-over are minimized by this systemic design difference. Other favorable characteristics of monolithic structures are preserved, such as low back pressure and high analyte mass transfer. In summary, the benefits of traditional polymeric monolithic media are conserved with the added benefits of economy and robustness.